The seedlings were then growing on the medium for 20 days . the primary results show that the tolerable concentration of kanamycin to the tested seedlings is up to 15ug / ml and the suggested concentration for selection of resistant plant is 25ug / ml 为筛选卡那霉素抗性的转基因植株,还进行了甘蓝型油菜卡那霉素敏感性实验。将正常甘蓝型油菜种子表面消毒后,接种于不同浓度卡那霉素ms琼脂培养基上,光照培养、筛选。